ACI Committee 363's ACI 363.2R-11 - Guide to Quality Control and Assurance of PDF

By ACI Committee 363

ISBN-10: 0870317032

ISBN-13: 9780870317033

High-strength concrete (HSC) has emerged as a workable fabric to exploit as a substitute to standard normal-strength concrete in infrastructure structures to lessen member pass part, expand member span size, lessen the variety of approach contributors, or increase method sustainability. This advisor bargains normal details at the quality controls and trying out of HSC. innovations are in line with the present kingdom of data won from around the world experimental study, analytical paintings, and box functions of HSC structures utilized in concrete buildings.

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Additional resources for ACI 363.2R-11 - Guide to Quality Control and Assurance of High-Strength Concrete

Sample text

8. ( B ) Possible pathway for the action of UDPGlcNAc 2-epimerase ( from Biochemistry 9, 885, 1970 ) . The DPN requirement indicated in the reaction scheme i s still entirely hypothetical. No enhancement of activity was observed on addition of DPN or TPN, but since many epimerases may contain tightly bound DPN, the postulated reaction mechanism is entirely plausible. The question whether one or two enzymes are involved in the epimeri­ zation has not been conclusively resolved by the studies of Salo and Fletcher, but the data support the view that the reaction is catalyzed by only one enzyme.

Evi­ dence to this effect was obtained from competition studies as well as from determinations of the activities toward the various substrates in mutants which had decreased levels of UDP-glucose 4-epimerase or lacked this enzyme entirely ( Maitra, 1971). D. UDP-N-ACETYLGLUCOSAMINE N-Acetylglucosamine I-phosphate + UTP � UDP-N-acetylglucosamine + PP, Formation of UDP-N-acetylglucosamine from N-acetylglucosamine 1phosphate is catalyzed by a pyrophosphorylase which appears to be dis­ tinct from UDP-glucose pyrophosphorylase, but the reaction has not been studied in any detail.

Furthermore, if proton re­ placement at C-2 occurred at the nucleotide sugar level, UDP-N-acetyl­ glucosamine and UDP-N-acetylmannosamine would be lab eled after ex­ posure to the 2-epimerase i n tritiated water. No radioactivity could be detected in the nucleotide sugars remaining after a short treatment with the enzyme, and the occurrence of a freely reversible interconversion b e­ tween UDP-N-acetylglucosamine and UDP-N-acetylmannosami ne as the fi rst step of the epimerase reaction is therefore clearly ruled out.

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ACI 363.2R-11 - Guide to Quality Control and Assurance of High-Strength Concrete by ACI Committee 363


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